Berberine chloride (Synonyms: 盐酸小檗碱; Natural Yellow 18 chloride)

目录号: HY-18258 纯度: 99.69%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Berberine chloride 是一种生物碱，常用作抗生素。Berberine chloride 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。抗肿瘤特性。

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Berberine chloride Chemical Structure

CAS No. : 633-65-8

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥429	In-stock
50 mg	￥390	In-stock
100 mg	￥660	In-stock
500 mg	￥1320	In-stock
1 g		询价
5 g		询价

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Other Forms of Berberine chloride:

MCE 顾客使用本产品发表的 45 篇科研文献

Proliferation Assay

Cell Viability Assay

: Berberine chloride purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]; Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 µM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.

: Berberine chloride purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]; Berberine (BBR; 2.5, 5, 10 µM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

: Berberine chloride purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]; Berberine (BBR; 2.5 µM; 10 days) significantly inhibits NCI-H441 colony formation.

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生物活性
纯度 & 产品资料
参考文献

生物活性

Berberine chloride is an alkaloid that acts as an antibiotic. Berberine chloride induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties^[1].

IC₅₀ & Target

ROS^[1]
DNA topoisomerase^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	21.84 μM Compound: Berberine	Cytotoxicity against mouse 3T3L1 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against mouse 3T3L1 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 28818460]
BHK-21	CC₅₀	> 100 μM Compound: 190	Cytotoxicity against BHK21 cells in presence of ATP by Celltiter-Glo assay Cytotoxicity against BHK21 cells in presence of ATP by Celltiter-Glo assay	[PMID: 28689975]
HeLa	IC₅₀	12.08 μg/mL Compound: Berberine	Cytotoxicity against human HeLa cells measured after 48 hrs by MTT assay Cytotoxicity against human HeLa cells measured after 48 hrs by MTT assay	[PMID: 31821990]
HeLa 229	IC₅₀	42.93 μM Compound: Berberine	Cytotoxicity against human HeLa 229 cells measured after 48 hrs by MTT assay Cytotoxicity against human HeLa 229 cells measured after 48 hrs by MTT assay	[PMID: 31821990]
Hep 3B2	IC₅₀	> 50 μM Compound: 1	Antiproliferative activity against human Hep3B cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human Hep3B cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 36640595]
HepG2	IC₅₀	> 50 μM Compound: 1	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 36640595]
HepG2	IC₅₀	> 50 μM Compound: 1	Antiproliferative activity against cisplatin-resistant human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against cisplatin-resistant human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 36640595]
HepG2	IC₅₀	46.9 μg/mL Compound: 1; BBR	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27561717]
Huh-7	IC₅₀	> 50 μM Compound: 1	Antiproliferative activity against human Huh-7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human Huh-7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 36640595]
HUVEC	IC₅₀	18.34 μM Compound: Berberine	Cytotoxicity against human HUVEC cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HUVEC cells assessed as reduction in cell viability by MTT assay	[PMID: 30660827]
LX-2	IC₅₀	> 50 μM Compound: 1	Antiproliferative activity against human LX2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human LX2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 36640595]
MDCK	CC₅₀	70.22 μg/mL Compound: Ber	Cytotoxicity against MDCK cells incubated for 48 hrs assessed as reduction in cell viability by SRB assay Cytotoxicity against MDCK cells incubated for 48 hrs assessed as reduction in cell viability by SRB assay	[PMID: 28958846]
RAW264.7	IC₅₀	> 30 μM Compound: 6	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay	[PMID: 22024033]
RAW264.7	IC₅₀	6.9 μM Compound: Berberine	Inhibition of PCSK9 mRNA level in mouse RAW264.7 cells by qRT-PCR assay Inhibition of PCSK9 mRNA level in mouse RAW264.7 cells by qRT-PCR assay	[PMID: 30698432]
SK-HEP1	IC₅₀	> 50 μM Compound: 1	Antiproliferative activity against human SK-HEP1 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human SK-HEP1 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 36640595]
SMMC-7721	IC₅₀	18.34 μM Compound: Berberine	Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay	[PMID: 30660827]
SW1990	IC₅₀	18.34 μM Compound: Berberine	Cytotoxicity against human SW1990 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human SW1990 cells assessed as reduction in cell viability by MTT assay	[PMID: 30660827]
Vero	IC₅₀	71.14 μg/mL Compound: Berberine	Cytotoxicity against African green monkey Vero cells measured after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells measured after 48 hrs by MTT assay	[PMID: 31821990]
Vero C1008	CC₅₀	221 μM Compound: 49	Cytotoxicity against African green monkey Vero E6 cells measured up to 24 to 72 hrs by MTT assay Cytotoxicity against African green monkey Vero E6 cells measured up to 24 to 72 hrs by MTT assay	[PMID: 31549836]

体外研究
(In Vitro)

Berberine (1.25-160 μM；72 hours) 对四种结直肠癌细胞系 LoVo、HCT116、SW480 和 HT-29 的增殖具有潜在的抑制作用^[1]。
Berberine (1.25-160 μM；24-72 小时) 诱导 LoVo 细胞生长的时间和剂量依赖性抑制^[1]。
LoVo 细胞暴露于小檗碱 (10 -80 μM) 24 小时。通过流式细胞术对 40 μM 小檗碱处理的 LoVo 细胞进行细胞周期分析显示细胞在 G2/M 期积累^[1]。
Berberine (10 -80 μM) 抑制细胞周期蛋白 B1，24 h 后 cdc2 和 cdc25c 蛋白表达，尤其是在80.0 μM 剂量下^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration:	1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:	72 hours
Result:	Inhibited the proliferation of four cell lines. The IC₅₀ ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay^[1]

Cell Line:	Colorectal carcinoma cell lines LoVo
Concentration:	1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:	24, 48, 72 hours
Result:	Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis^[1]

Cell Line:	LoVo cells
Concentration:	0, 10, 20, 40, or 80 μM
Incubation Time:	24 hours
Result:	Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis^[1]

Cell Line:	LoVo cells
Concentration:	10, 20, 40, or 80 μM
Incubation Time:	24 hours
Result:	Suppressed cyclin B1, cdc2 and cdc25c protein expression.

体内研究
(In Vivo)

Berberine (10、30 或 50 mg/kg/天；胃肠道强饲法；连续 10 天) 在体内抑制人结直肠腺癌的生长。胃肠道灌胃剂量为 30 和 50 mg/kg/day 的小檗碱对人结直肠腺癌裸鼠异种移植物生长的抑制率分别为 33.1% 和 45.3%^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts^[1]
Dosage:	10, 30, or 50 mg/kg/day
Administration:	Gastrointestinal gavage; for 10 consecutive days
Result:	Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.

Clinical Trial

分子量

371.81

Formula

C₂₀H₁₈ClNO₄

CAS 号

633-65-8

性状

固体

颜色

Light yellow to yellow

中文名称

盐酸小檗碱；盐酸黄连素；盐酸黄莲素

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 8.33 mg/mL (22.40 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.6895 mL	13.4477 mL	26.8955 mL
5 mM	0.5379 mL	2.6895 mL	5.3791 mL
10 mM	0.2690 mL	1.3448 mL	2.6895 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.36 mM); 澄清溶液

此方案可获得 ≥ 1.25 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.36 mM); 澄清溶液

此方案可获得 ≥ 1.25 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 0.5% CMC-Na/saline water
Solubility: 11 mg/mL (29.59 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.69%

选择批次：

Data Sheet (649 KB) SDS (393 KB)

COA (289 KB) LCMS (95 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Cai Y, et al. Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo. J Nat Med. 2014 Jan;68(1):53-62. [Content Brief]

Berberine chloride 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6895 mL	13.4477 mL	26.8955 mL	67.2386 mL
	5 mM	0.5379 mL	2.6895 mL	5.3791 mL	13.4477 mL
	10 mM	0.2690 mL	1.3448 mL	2.6895 mL	6.7239 mL
	15 mM	0.1793 mL	0.8965 mL	1.7930 mL	4.4826 mL
	20 mM	0.1345 mL	0.6724 mL	1.3448 mL	3.3619 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Berberine633-65-8Natural Yellow 18盐酸小檗碱盐酸黄连素盐酸黄莲素Natural Yellow18Natural Yellow-18TopoisomeraseAutophagyBacterialReactive Oxygen SpeciesAntibioticEndogenous MetaboliteParasiteInhibitorinhibitorinhibit

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Berberine chloride (Synonyms: 盐酸小檗碱; Natural Yellow 18 chloride)

Customer Review

Other Forms of Berberine chloride:

MCE 顾客使用本产品发表的 45 篇科研文献

Berberine chloride 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Topoisomerase 亚型特异性产品:

查看 Antibiotic 亚型特异性产品:

查看 Parasite 亚型特异性产品:

生物活性

纯度 & 产品资料

参考文献

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Berberine chloride 相关分类

完整储备液配制表

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Berberine chloride (Synonyms: 盐酸小檗碱; Natural Yellow 18 chloride)

Customer Review

Other Forms of Berberine chloride:

Berberine chloride 相关抗体

MCE 顾客使用本产品发表的 45 篇科研文献

Berberine chloride 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Topoisomerase 亚型特异性产品:

查看 Antibiotic 亚型特异性产品:

查看 Parasite 亚型特异性产品:

生物活性

纯度 & 产品资料

参考文献

Berberine chloride 相关抗体:

摩尔计算器

稀释计算器

Berberine chloride 相关分类

完整储备液配制表

Keywords:

您最近查看的产品:

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