Palmatine chloride is an orally active and irreversible indoleamine 2,3-dioxygenase 1 (IDO-1) inhibitor with IC50s of 3 μM and 157μM against HEK 293-hIDO-1 and rhIDO-1, respectively. Palmatine chloride can also inhibit West Nile virus (WNV) NS2B-NS3 protease in an uncompetitive manner with an IC50 of 96 μM. Palmatine chloride shows anti-cancer, anti-oxidation, anti-inflammatory, neuroprotection, antibacterial, anti-viral activities[1][2][3][4][5].
Palmatine (0-100 μM; 42 h) suppresses WNV with an EC50 value of 3.6 μM, and reduce the viral titers of DENV-2 and YFV with EC50 values of 26.4 μM and 7.3 μM, respectively[3]. ?
Palmatine (0-1128 μM; 24-72 h) inhibits colon cancer cell proliferation[5]. ?
Palmatine (0-704 μM; 24 h) reduces AURKA protein levels, induces G2/M phase arrest, and induces apoptosis in colon cancer cells via the mitochondrial associated pathway[5].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Promoted the expression of apoptosis markers such as P53 / P73, Caspase3, and Caspase9. Reduced AURKA protein levels. Increased cyt. c in the cytoplasm while reduced Bcl2 and Bcl-xl in a dose-dependent manner.
Palmatine (50 or 100 mg/kg; p.o.; daily for 7 days) ameliorates DSS (dextran sulfate sodium)-induced colitis and prevents infiltration of inflammatory cells[1].?
Palmatine (0-200 mg/kg; i.p.; once) attenuates D-galactosamine/Lipopolysaccharides (HY-D1056)-induced fulminant hepatic failure in mice[2].?
Palmatine (0-1 mg/kg; i.p.; 10 days) shows memory-enhancing activity in mice[4].?
Palmatine (33.75-135 mg/kg; p.o.; daily for 26 days) can effectively inhibit the growth of HCT-116 xenografts in mice[5].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
DSS- induced Colitis BALB/c mice model (8-week-old)[1]
Dosage:
50 or 100 mg/kg
Administration:
Orally, daily, for 7 days
Result:
Ameliorated DSS-induced colitis and prevented infiltration of inflammatory cells; remarkably extended the colon length; significantly suppressed the colonic MPO activity. Decreased the levels of colonic inflammatory cytokines (TNF-α, IFN-γ, IL-1β, IL-6, IL-4 and IL-10); Protected mucosal integrity by modulating TJs protein and apoptosis proteins; Restored DSS-induced decreases of TJ protein ZO-1, ZO-2 and claudin-1; Reduced Bax expression and enhanced Bcl-2 expression at the dose of 100 mg/kg, prevented epithelial apoptosis and improved intestinal integrity. Prevented DSS-induced changes of gut microbiota in colitis mice.
Intraperitoneal injection, 1 h before the GalN/LPS treatment
Result:
Attenuated the mortality and serum aminotransferase activities increased by GalN/LPS. Prevented the increase of serum TNF-α and augmented that of serum IL-10. Decreased the TNF-a mRNA expression and increased the IL-10 mRNA expression. Attenuated the apoptosis of hepatocytes.
Animal Model:
Swiss young male albino mice, with Scopolamine (HY-N0296)- and diazepam-induced amnesia model[4]
Dosage:
0.1, 0.5, 1 mg/kg
Administration:
Intraperitoneal injection, 10 days
Result:
Significantly improved learning and memory of mice at 0.5 and 1 mg/kg and did not show any significant effect on locomotor activity of the mice. Significantly reversed scopolamine- and diazepam-induced amnesia in mice. Significantly reduced brain acetylcholinesterase activity of mice.
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。
可选溶剂
浓度溶剂体积质量
1 mg
5 mg
10 mg
25 mg
H2O / DMSO
1 mM
2.5782 mL
12.8912 mL
25.7825 mL
64.4562 mL
5 mM
0.5156 mL
2.5782 mL
5.1565 mL
12.8912 mL
10 mM
0.2578 mL
1.2891 mL
2.5782 mL
6.4456 mL
DMSO
15 mM
0.1719 mL
0.8594 mL
1.7188 mL
4.2971 mL
20 mM
0.1289 mL
0.6446 mL
1.2891 mL
3.2228 mL
25 mM
0.1031 mL
0.5156 mL
1.0313 mL
2.5782 mL
30 mM
0.0859 mL
0.4297 mL
0.8594 mL
2.1485 mL
40 mM
0.0645 mL
0.3223 mL
0.6446 mL
1.6114 mL
50 mM
0.0516 mL
0.2578 mL
0.5156 mL
1.2891 mL
60 mM
0.0430 mL
0.2149 mL
0.4297 mL
1.0743 mL
80 mM
0.0322 mL
0.1611 mL
0.3223 mL
0.8057 mL
*
备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。
Help & FAQs
Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Powered by Bioz
