2. Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease
  3. COX Ferroptosis Endogenous Metabolite
  4. (-)-Epicatechin

(-)-Epicatechin  (Synonyms: 表儿茶素; (-)-Epicatechol; Epicatechin; epi-Catechin)

目录号: HY-N0001 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

(-)-Epicatechin 抑制环加氧酶-1 (COX-1) , IC50为 3.2 μM。(-)-Epicatechin 通过阻断 NF-κB 的 p65 亚基的核定位来抑制 IL-1β 诱导的 iNOS 表达。

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(-)-Epicatechin Chemical Structure

(-)-Epicatechin Chemical Structure

CAS No. : 490-46-0

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥770
In-stock
1 mg ￥218
In-stock
5 mg ￥437
In-stock
10 mg ￥700
In-stock
50 mg ￥1100
In-stock
100 mg ￥1600
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of (-)-Epicatechin:

(-)-Epicatechin 相关抗体

Top Publications Citing Use of Products

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COX COX-1 COX-2 COX-3

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

(-)-Epicatechin inhibits cyclooxygenase-1 (COX-1) with an IC50 of 3.2 μM. (-)-Epicatechin inhibits the IL-1β-induced expression of iNOS by blocking the nuclear localization of the p65 subunit of NF-κB.

IC50 & Target[1]

COX-1

3.2 μM (IC50)

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
BMDC IC50
> 50 μM
Compound: 17
Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
[PMID: 25769817]
BMDC IC50
> 50 μM
Compound: 17
Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
[PMID: 25769817]
BMDC IC50
> 50 μM
Compound: 17
Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
[PMID: 25769817]
BV-2 IC50
47.37 μM
Compound: 11
Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction
Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction
[PMID: 27623545]
HCT-8 ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
KB ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
L929 EC50
300 μM
Compound: epicatechin
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining
[PMID: 9287415]
L929 EC50
350 μM
Compound: epicatechin
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
[PMID: 9287415]
LNCaP EC50
13 μM
Compound: 1
Decrease in androgen receptor protein expression in LNCaP cells by Western blotting
Decrease in androgen receptor protein expression in LNCaP cells by Western blotting
[PMID: 17383188]
MT4 CC50
136.2 μg/mL
Compound: 11
Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
[PMID: 10425115]
MT4 IC50
34.1 μg/mL
Compound: 11
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
[PMID: 10425115]
PC-3 IC50
> 500 μM
Compound: 2
In vitro inhibitory concentration against PC-3 cell line in MTT assay was determined after 3 days incubation
In vitro inhibitory concentration against PC-3 cell line in MTT assay was determined after 3 days incubation
[PMID: 15380225]
Platelet IC50
> 50 μg/mL
Compound: (-)-epi-Catechin
Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry
Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry
[PMID: 21106454]
Platelet IC50
22.22 μg/mL
Compound: (-)-epi-Catechin
Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry
Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry
[PMID: 21106454]
SK-OV-3 IC50
> 500 μM
Compound: 2
In vitro inhibitory concentration against SKOV3 cell line in MTT assay was determined after 3 days incubation
In vitro inhibitory concentration against SKOV3 cell line in MTT assay was determined after 3 days incubation
[PMID: 15380225]
TE-671 ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
U-373MG ATCC IC50
> 500 μM
Compound: 2
In vitro inhibitory concentration against U373MG cell line in MTT assay was determined after 3 days incubation
In vitro inhibitory concentration against U373MG cell line in MTT assay was determined after 3 days incubation
[PMID: 15380225]
Vero IC50
184.1 μM
Compound: 5
Cytotoxicity against african green monkey Vero cells after 4 hrs by MTT assay
Cytotoxicity against african green monkey Vero cells after 4 hrs by MTT assay
[PMID: 16724851]
体外研究
(In Vitro)

(-)-Epicatechin 在 70 μg/mL 时对环氧合酶-1 (COX-1) 表现出 >95% 的抑制活性，IC50 为 3.2 μM[1]。
(-)-Epicatechin 通过阻断 NF-κB 的 p65 亚基的核定位来抑制 IL-1β 诱导的 iNOS 表达。在 RINm5F 细胞中，(-)- Epicatechin 显示可阻断添加 IL-1β 后胰岛素释放的抑制。此外，(-)-Epicatechin 显示可抑制霍奇金淋巴瘤细胞和 Jurkat T 细胞的增殖，这归因于 (-)-Epicatechin 抑制这些细胞中 NF-κB 与 DNA 结合的能力。在人结直肠癌 HCT-116 细胞中，将 20 μM Panaxadiol 与 150、200 或 250 μM (-)-Epicatechin 结合使用可分别导致 51%、97% 和 95% 的生长抑制。如膜联蛋白 V/PI 染色所检测，该组合还将细胞凋亡水平提高了 11.9%、16.6% 和 25.8%[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

(-)-Epicatechin 相关抗体:
体内研究
(In Vivo)

动物通过口服强饲法 (每天两次) 接受 1 mg/kg 的 (-)-Epicatechin 或水 (载体)。运动组进行 15 天的跑步机运动。使用 (-)-Epicatechin 可显著提高跑步机性能 (~50%) 和增强原位肌肉抗疲劳性 (~30%)[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

290.27

Formula

C15H14O6
CAS 号
性状

固体

颜色

White to light yellow

中文名称

表儿茶素；(-)-表儿茶素；表儿茶精；表兒茶酚
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (344.51 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : 2 mg/mL (6.89 mM; 超声助溶 (<60°C))

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.4451 mL 17.2253 mL 34.4507 mL
5 mM 0.6890 mL 3.4451 mL 6.8901 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.61 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.61 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.00%

COA (287 KB) LCMS (147 KB)

产品使用指南 (1538 KB)
参考文献
Animal Administration
[3]

Mice[3]
1-year-old, male C57BL/6N mice (n=25) are randomized into four groups. Mice in the (-)-Epicatechin groups 3 and 4 are given 1.0 mg/kg twice a day (morning and evening) for 15 consecutive days, whereas animals in the control groups 1 and 2 receive the vehicle (water). Both (-)-Epicatechin and vehicle are administered via oral gavage[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

(-)-Epicatechin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.4451 mL 17.2253 mL 34.4507 mL 86.1267 mL
5 mM 0.6890 mL 3.4451 mL 6.8901 mL 17.2253 mL
DMSO 10 mM 0.3445 mL 1.7225 mL 3.4451 mL 8.6127 mL
15 mM 0.2297 mL 1.1484 mL 2.2967 mL 5.7418 mL
20 mM 0.1723 mL 0.8613 mL 1.7225 mL 4.3063 mL
25 mM 0.1378 mL 0.6890 mL 1.3780 mL 3.4451 mL
30 mM 0.1148 mL 0.5742 mL 1.1484 mL 2.8709 mL
40 mM 0.0861 mL 0.4306 mL 0.8613 mL 2.1532 mL
50 mM 0.0689 mL 0.3445 mL 0.6890 mL 1.7225 mL
60 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4354 mL
80 mM 0.0431 mL 0.2153 mL 0.4306 mL 1.0766 mL
100 mM 0.0345 mL 0.1723 mL 0.3445 mL 0.8613 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Epicatechin490-46-0(-)-Epicatechol Epicatechin epi-Catechin表儿茶素(-)-表儿茶素表儿茶精表兒茶酚COXFerroptosisEndogenous MetaboliteCyclooxygenaseInhibitorinhibitorinhibit

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